![]() Megapixels: Uvitec’s system has a 9.2 native megapixel camera, and the included software can create an enhanced image with 18 megapixels.MIDSCI proudly recommends the Uvitec Alliance Q9 Advanced gel documentation system for its performance in each of these four categories: The Industry’s Leading Gel Documentation System: Uvitec Alliance Q9 Cooling the camera reduces the level of noise per unit of time.īoth specifications aid in increasing signal-to-noise ratio.A wider lens aperture reduces the amount of time required to gather the desired level of signal.These camera specs work together to increase sensitivity of an image because: Note: Sensor temperature and lens aperture have a combined effect on signal-to-noise ratio. The effect of aperture value in revealing faint bandscan be seen in the image below: And while competing systems have a low ratio value of F/0.9 or higher, the lowest aperture of our leading system is F/0.8. The lowest aperture ratio is the standard setting for Western blots. In other words, lower aperture increases a camera’s sensitivity, and reduces the time needed to reach desired signal level.Īperture is controlled by an iris and, for most imaging systems, several aperture ratios can be selected. The lower this ratio, the more photons can pass through the lens to the detector. Lens aperture is defined as the ratio of focal length to effective aperture diameter. The effect of deep cooling on noise reduction can be seen in the 3D images below: For example, ambient -50 o C is only -25 o C, or 35 degrees higher than our product. Our leading product cools to -60 o Celsius for reduced noise, whereas many competing systems express cooling from ambient at a much higher temperature. Reducing the sensor’s temperature decreases the dark noise. These thermally generated electrons create noise in the detector. This creates the desired signal.ĭark noise is the generation of thermal electrons at a given CCD temperature. A current is created when a photon strikes the CCD sensor. #3: Sensor Temperature andĬooling a CCD is important because this will decrease noise - which, in turn, increases sensitivity. The larger the bit depth of a camera, the greater the ability to quantify strong versus weak bands of DNA. Once a pixel is exposed to maximum intensity, it can no longer be used for quantification. By comparison, a 16-bit camera can measure 65,535 levels of intensity, with a dynamic range of 4.8. A 12-bit camera can measure 4,096 levels of intensity. The image below explains the concept of binning:īit depth is the number of bits devoted to counting the intensity of light of an individual pixel in an image. By creating a large pixel out of a 2x2 pixel box, sensitivity is four times greater, and the resolution or number of pixels is four times less. For this reason, the number of native megapixels is an important number to compare: While an enhanced image may look better to the eye, it does not increase actual sensitivity or resolution of the data.īinning combines several pixels to make a larger, virtual CCD to increase sensitivity. The number of pixels in an image taken by the camera can be digitally enhanced or extended - to create more pixels in the image, and to make the image more appealing to the eye. Native megapixels are the number of pixels in a CCD camera. As with consumer cameras, a greater pixel count produces images with higher resolution.īelow is an example of how resolution improves by increasing the number of pixels: When evaluating a system, these are the four most important features of a gel imager’s CCD camera:Ĭamera Specifications Can Affect Your Western Blot Imaging: #1: The Number of Native Megapixels.Ī megapixel number (mp) represents the number of individual points in an image. The CCD camera is the heart of a gel documentation system and understanding the specifications of the camera system can help you find the best option for your needs. Nowadays, a gel documentation system (also referred to as a “gel doc” or “gel imager,”) with a digital camera and sophisticated software has made the process much easier, rapid, and flexible. This research started with lab notebooks being filled with images that were created by exposing and developing film. Researchers have long since relied on gel documentation in protein, DNA, and RNA research. The 4 Most Important Camera Specs of a Gel Documentation System The 4 Most Important Camera Specs of a Gel Documentation SystemĮvery industry has harnessed the power of improved technology to address old pain points and streamline efficiency - and gel documentation is no exception. Stoolfix Gut Microbiome Stabilization Kits.Beakers, Graduated Cylinders, and Flasks.Aluminum Foil, Parafilm, and Multiwipes.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |